منابع مشابه
Studies on Succinic Dehydrogenase
About 2 years ago the author reported that the activity of purified preparations of succinic dehydrogenase is enhanced by phosphate buffer (2). This enhancement was dependent upon the phosphate concentration and appeared to be relatively specific for phosphate, since other buffers, such as Tris,l imidazole, histidine, glycylglycine, and arsenate did not induce it (2, 3). Arsenate, in fact, inte...
متن کاملSuccinic Semialdehyde Dehydrogenase
The enzyme succinic semialdehyde dehydrogenase from pig brain has been 2000-fold purified by a combination of DEAE-cellulose, hydroxyapatite, and AMPSepharose chromatography. This preparation has a molecular weight of 160,000 and a specific activity of 5.3 pmol/min*mg at 25°C. The inhibition of succinic semialdehyde dehydrogenase by carbonyl compounds, i.e. P-pyridoxal and o-phthalaldehyde was ...
متن کاملSuccinic dehydrogenase of Azotobacter vinelandii.
During the investigation of the Krebs cycle in Azotobacter agile, strain 4.4 (Repaske and Wilson, 1953), it was found that whole cells normally required 20 minutes before adaptation to succinate, but succinoxidase activity could be demonstrated in cell-free extracts made from unadapted cells. If extracts prepared in a 10 kc Raytheon sonic oscillator were centrifuged at 145,000 G for 30 minutes,...
متن کاملInhibition of succinic dehydrogenase by oxalacetate.
Although the inhibition of succinic dehydrogenase by oxalacetate is a generally accepted fact, there is a paucity of data on the subject. In 1937 Das (l), using a modified Thunberg technique, reported that the enzyme was 50 per cent inhibited by 2 X 1O-5 M oxalacetate when the succinate concentration was 0.025 M. In 1939 Potter (2) reported that the oxidation of succinate by liver and kidney ho...
متن کاملInhibition of succinic dehydrogenase by methylglyoxal.
The reaction of the carbonyl group of methylglyoxal with -SH radicals of thiols has been the subject of numerous investigations (l-9). The reactivity of the keto aldehyde with -SH compounds suggested that a similar type of reaction may occur between methylglyoxal and -SH proteins. The physiological r&e of methylglyoxal as an inhibitor of -SK enzymes is of particular interest, since this keto al...
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ژورنال
عنوان ژورنال: Nature
سال: 1947
ISSN: 0028-0836,1476-4687
DOI: 10.1038/159374a0